ABSTRACT
Objetivo: Desenvolver esferas de quitosana/D. ambrosioides (mastruz) para aplicação como biomaterial. Material e Métodos: Para obtenção das esferas de quitosana e quitosana com extrato alcoólico bruto do mastruz (EAB) utilizou-se a técnica de GeleificaçãoIonotrópica. Foram formados quatro grupos: QO- quitosana; Q5; Q10 e Q20, quitosana com 5%, 10% e 20% de EAB, respectivamente. Foram caracterizadas por Microscopia Óptica (MO); Citotoxicidade e Biodegradação. Resultados: NoMO, observou-se esferas bem delimitadas e mais escuras, à medida que o percentual do EAB aumentou. Todos os grupos foram não citotóxicos. Na presença de lisozima, observou-se perda de massa, já na solução tampão fosfato salina (PBS) constatou-se ganho de massa. Conclusão: Conclui-se que as esferas apresentaram potencial para utilização como biomaterial.
Objective: This worked aimed to develop chitosan/D. ambrosioides (mastruz) spheres to be applied as a biomaterial. Material and Methods: To obtain the chitosan spheres and the chitosan with crude ethanolic extract of mastruz (CEE) the Ionotropic gelation technique was used. Four groups were formed: QO- Chitosan; Q5; Q10 and Q20, Chitosan with 5%, 10% and 20% of CEE, respectively. Were characterized by Optic Microscopy (OM); Cytotoxicity and Biodegradation.Results: InOM, well delimited and darker spheres were observed, as the CEE percentage increased. All groups were noncytotoxic. In the presence of lysozyme, mass loss was observed, as for in solution Phosphate Buffered Saline (PBS) the mass gain was found. Conclusion: It can be concluded that the spheres presented potential for use as a biomaterial.
ABSTRACT
Trichophyton mentagrophytes is a fungus causative agent of dermatophytosis, affecting humans worldwide. This has driven the search for products for the treatment of these infections. Accordingly, the aim of this study was to investigate the antifungal activity of the Cymbopogon winterianus essential oil against T. mentagrophytes. The antifungal tests consisted of antifungal screening, determination of MIC and MFC, analysis of the essential oil's effects on mycelial growth, germination of fungal spores, fungal viability, morphogenesis, cell wall (test with sorbitol) and cell membrane (cell leakage test) of T. mentagrophytes. Upon screening, the oil inhibited all strains, with zones of growth inhibition of 24-28 mm in diameter. The MIC was 312 μg/mL and CFM was 2500 μg/mL for almost all the strains tested. There were morphological changes in the conidia group, form and pigmentation of hyphae. The antifungal action of the product does not involve the cell wall and its action may involve the fungal plasma membrane. It is concluded that C. winterianus essential oil constitutes a potential antifungal product, especially for the treatment of dermatophytosis.
Trichophyton mentagrophytes é um fungo causador de dermatofitoses, afetando humanos em todo o mundo. Isto direciona a busca de produtos para o tratamento destas infecções. Assim, este estudo teve por objetivo investigar a atividade antifúngica do óleo essencial de Cymbopogon winterianus contra T. mentagrophytes. Os ensaios antifúngicos foram constituídos do screening antifúngico, da determinação CIM e CFM, da análise dos efeitos do óleo essencial no crescimento micelial, na germinação dos esporos, na viabilidade fúngica, na morfogênese, na parede celular (ensaio com sorbitol) e na membrana celular (ensaio de lise celular) de T. mentagrophytes. No screening, o óleo inibiu todas as cepas, com zonas de inibição de crescimento de 24-28 mm de diâmetro. A CIM foi de 312 μg/mL e a CFM foi de 2500 μg/mL para quase todas as cepas testadas. O óleo essencial inibiu o desenvolvimento micelial, a germinação dos esporos e a viabilidade fúngica. Houve alterações morfológicas no agrupamento dos conídios, na forma e pigmentação das hifas. A ação antifúngica do produto não envolve a parede celular e parece estar envolvida com a membrana celular fúngica. Pode-se concluir que o óleo essencial de C. winterianus se apresenta como um potencial produto antifúngico, especialmente para o tratamento das dermatofitoses.
Subject(s)
Cymbopogon , Oils, Volatile/analysis , Trichophyton , Trichophyton/isolation & purification , Trichophyton/pathogenicity , Antifungal Agents/analysis , Antifungal Agents/pharmacology , Antifungal Agents/isolation & purification , Dermatomycoses , MycologyABSTRACT
Trichophyton rubrum is one of the most common fungi causer of dermatophytosis, mycosis that affect humans and animals around the world. Researches aiming new products with antifungal activity become necessary to overcome difficulties on treatment of these infections. Accordingly, this study aimed to investigate the antifungal activity of essential oil from Cymbopogon winterianus against the dermatophyte T. rubrum. The antifungal screening was performed by solid medium diffusion method with 16 T. rubrum strains, minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined using the microdilution method. The effects on mycelial dry weight and morphology were also observed. Screening showed essential oil in natura inhibited all the tested strains, with inhibition zones between 24-28 mm diameter. MIC50 and MIC90 values of the essential oil were 312 µg/mL for nearly all the essayed strains (93.75 percent) while the MFC50 and MFC90 values were about eight times higher than MIC for all tested strains. All tested essential oil concentrations managed to inhibit strongly the mycelium development. Main morphological changes on the fungal strains observed under light microscopy, which were provided by the essential oil include loss of conidiation, alterations concerning form and pigmentation of hyphae. In the oil presence, colonies showed folds, cream color and slightly darker than the control, pigment production was absent on the reverse and with evident folds. It is concluded that C. winterianus essential oil showed activity against T. rubrum. Therefore, it could be known as potential antifungal compound especially for protection against dermatophytosis.